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Chimeric padlock and iLock probes for increased efficiency of targeted RNA detection
Many approaches exist to detect RNA using complementary oligonucleotides. DNA ligation-based techniques can improve discrimination of subtle sequence variations, but they have been difficult to implement for direct RNA analysis due to the infidelity and inefficiency of most DNA ligases on RNA. In th...
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| Veröffentlicht in: | RNA |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Cold Spring Harbor Laboratory Press
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6298565/ https://ncbi.nlm.nih.gov/pubmed/30309880 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.066753.118 |
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