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Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators

Prokaryotic Argonaute proteins (pAgos) have been proposed as an alternative to the CRISPR/Cas9 platform for gene editing. Although Argonaute from Natronobacterium gregoryi (NgAgo) was recently shown unable to cleave genomic DNA in mammalian cells, the utility of NgAgo or other pAgos as a targetable...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: O’Geen, Henriette, Ren, Chonghua, Coggins, Nicole B., Bates, Sofie L., Segal, David J.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5870970/
https://ncbi.nlm.nih.gov/pubmed/29584750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0193818
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