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Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators

Prokaryotic Argonaute proteins (pAgos) have been proposed as an alternative to the CRISPR/Cas9 platform for gene editing. Although Argonaute from Natronobacterium gregoryi (NgAgo) was recently shown unable to cleave genomic DNA in mammalian cells, the utility of NgAgo or other pAgos as a targetable...

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Foilsithe in:PLoS One
Main Authors: O’Geen, Henriette, Ren, Chonghua, Coggins, Nicole B., Bates, Sofie L., Segal, David J.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Public Library of Science 2018
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5870970/
https://ncbi.nlm.nih.gov/pubmed/29584750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0193818
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