Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

Cell-free protein synthesis has emerged as a powerful approach for expanding the range of genetically encoded chemistry into proteins. Unfortunately, efforts to site-specifically incorporate multiple non-canonical amino acids into proteins using crude extract-based cell-free systems have been limite...

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Udgivet i:Nat Commun
Main Authors: Martin, Rey W., Des Soye, Benjamin J., Kwon, Yong-Chan, Kay, Jennifer, Davis, Roderick G., Thomas, Paul M., Majewska, Natalia I., Chen, Cindy X., Marcum, Ryan D., Weiss, Mary Grace, Stoddart, Ashleigh E., Amiram, Miriam, Ranji Charna, Arnaz K., Patel, Jaymin R., Isaacs, Farren J., Kelleher, Neil L., Hong, Seok Hoon, Jewett, Michael C.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5865108/
https://ncbi.nlm.nih.gov/pubmed/29572528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-03469-5
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