Subnanogram proteomics: impact of LC column selection, MS instrumentation and data analysis strategy on proteome coverage for trace samples

One of the greatest challenges for mass spectrometry (MS)-based proteomics is the limited ability to analyze small samples. Here we investigate the relative contributions of liquid chromatography (LC), MS instrumentation and data analysis methods with the aim of improving proteome coverage for sampl...

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Detalhes bibliográficos
Publicado no:Int J Mass Spectrom
Main Authors: Zhu, Ying, Zhao, Rui, Piehowski, Paul D., Moore, Ronald J., Lim, Sujung, Orphan, Victoria J., Paša-Tolić, Ljiljana, Qian, Wei-Jun, Smith, Richard D., Kelly, Ryan T.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5863755/
https://ncbi.nlm.nih.gov/pubmed/29576737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ijms.2017.08.016
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