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Deep and Highly Sensitive Proteome Coverage by LC-MS/MS Without Prefractionation

In-depth MS-based proteomics has necessitated fractionation of either proteins or peptides or both, often requiring considerable analysis time. Here we employ long liquid chromatography runs with high resolution coupled to an instrument with fast sequencing speed to investigate how much of the prote...

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Main Authors: Thakur, Suman S., Geiger, Tamar, Chatterjee, Bhaswati, Bandilla, Peter, Fröhlich, Florian, Cox, Juergen, Mann, Matthias
格式: Artigo
語言:Inglês
出版: The American Society for Biochemistry and Molecular Biology 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3149084/
https://ncbi.nlm.nih.gov/pubmed/21586754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M110.003699
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