Deep and Highly Sensitive Proteome Coverage by LC-MS/MS Without Prefractionation

In-depth MS-based proteomics has necessitated fractionation of either proteins or peptides or both, often requiring considerable analysis time. Here we employ long liquid chromatography runs with high resolution coupled to an instrument with fast sequencing speed to investigate how much of the prote...

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Bibliographische Detailangaben
Hauptverfasser: Thakur, Suman S., Geiger, Tamar, Chatterjee, Bhaswati, Bandilla, Peter, Fröhlich, Florian, Cox, Juergen, Mann, Matthias
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Biochemistry and Molecular Biology 2011
Schlagworte:
Technological Innovation and Resources
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3149084/
https://ncbi.nlm.nih.gov/pubmed/21586754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M110.003699
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