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Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins
[Image: see text] Here, we report the site-specific incorporation of a thioester containing noncanonical amino acid (ncAA) into recombinantly expressed proteins. Specifically, we genetically encoded a thioester-activated aspartic acid (ThioD) in bacteria in good yield and with high fidelity using an...
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| Publicado no: | ACS Chem Biol |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Chemical
Society
2018
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5856652/ https://ncbi.nlm.nih.gov/pubmed/29360343 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.7b00998 |
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