CRISPR RNAs trigger innate immune responses in human cells

Here, we report that CRISPR guide RNAs (gRNAs) with a 5′-triphosphate group (5′-ppp gRNAs) produced via in vitro transcription trigger RNA-sensing innate immune responses in human and murine cells, leading to cytotoxicity. 5′-ppp gRNAs in the cytosol are recognized by DDX58, which in turn activates...

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Detalhes bibliográficos
Publicado no:Genome Res
Main Authors: Kim, Sojung, Koo, Taeyoung, Jee, Hyeon-Gun, Cho, Hee-Yeon, Lee, Gyeorae, Lim, Dong-Gyun, Shin, Hyoung Shik, Kim, Jin-Soo
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2018
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5848615/
https://ncbi.nlm.nih.gov/pubmed/29472270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.231936.117
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