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Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes

Over the past few years, tools that make use of the Cas9 nuclease have led to many breakthroughs, including in the control of gene expression. The catalytically dead variant of Cas9 known as dCas9 can be guided by small RNAs to block transcription of target genes, in a strategy also known as CRISPRi...

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Détails bibliographiques
Publié dans:Mol Syst Biol
Auteurs principaux: Vigouroux, Antoine, Oldewurtel, Enno, Cui, Lun, Bikard, David, van Teeffelen, Sven
Format: Artigo
Langue:Inglês
Publié: John Wiley and Sons Inc. 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5842579/
https://ncbi.nlm.nih.gov/pubmed/29519933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/msb.20177899
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