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A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to decipher gene functions and genetic interactions. Here we use a genome-wide library of guide RNAs to direct the catalytically dead Cas9 (dCas9) to block gene transcription in Escherichia coli. Using a machine-learning app...
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| Veröffentlicht in: | Nat Commun |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group UK
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5954155/ https://ncbi.nlm.nih.gov/pubmed/29765036 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-04209-5 |
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