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In-cell production of a genetically-encoded library based on the θ-defensin RTD-1 using a bacterial expression system
We report the high-yield heterologous expression of bioactive θ-defensin RTD-1 inside Escherichia coli cells by making use of intracellular protein trans-splicing in combination with a high efficient split-intein. RTD-1 is a small backbone-cyclized polypeptide with three disulfide bridges and a natu...
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| Publicado en: | Bioorg Med Chem |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2017
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5840032/ https://ncbi.nlm.nih.gov/pubmed/28927803 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmc.2017.09.002 |
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