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In-cell production of a genetically-encoded library based on the θ-defensin RTD-1 using a bacterial expression system

We report the high-yield heterologous expression of bioactive θ-defensin RTD-1 inside Escherichia coli cells by making use of intracellular protein trans-splicing in combination with a high efficient split-intein. RTD-1 is a small backbone-cyclized polypeptide with three disulfide bridges and a natu...

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Detalhes bibliográficos
Publicado no:Bioorg Med Chem
Main Authors: Bi, Tao, Li, Yilong, Shekhtman, Alexander, Camarero, Julio A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5840032/
https://ncbi.nlm.nih.gov/pubmed/28927803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmc.2017.09.002
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