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Theory and Application of the Relationship between Steady-State Isotope Effects on Enzyme Intermediate Concentrations and Net Rate Constants()

Steady-state kinetic isotope effects on enzyme catalyzed reactions are often interpreted in terms of the microscopic rate constants associated with the elementary reactions of interest. Unfortunately, this approach can lead to confusion, especially when more than one elementary reaction is isotopica...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Ruszczycky, Mark W., Liu, Hung-wen
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5837895/
https://ncbi.nlm.nih.gov/pubmed/28911781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2017.07.022
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