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Spontaneous CRISPR loci generation in vivo by non-canonical spacer integration

The adaptation phase of CRISPR-Cas immunity depends on the precise integration of short segments of foreign DNA (spacers) into a specific genomic location within the CRISPR locus by the Cas1-Cas2 integration complex. Although off-target spacer integration outside of canonical CRISPR arrays has been...

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Bibliografiske detaljer
Udgivet i:Nat Microbiol
Main Authors: Nivala, Jeff, Shipman, Seth L., Church, George M.
Format: Artigo
Sprog:Inglês
Udgivet: 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5832444/
https://ncbi.nlm.nih.gov/pubmed/29379209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41564-017-0097-z
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