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Spontaneous excision and facilitated recovery as a control for phenotypes arising from RNA interference and other dominant transgenes
An essential control for genetic manipulation of microbes is the regeneration of the wild-type state and phenotype to validate that any mutant phenotypes are ‘on target’. For Leishmania gene knockouts, this is often done by re-expression of the target gene from episomal vectors, often bearing counte...
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| Publicado no: | Mol Biochem Parasitol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5828908/ https://ncbi.nlm.nih.gov/pubmed/29357296 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2018.01.004 |
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