i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases

We present a robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation. The method generates mouse models containing single-base changes, kilobase-sized deletions, and knock-ins. The...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Genome Biol
Main Authors: Ohtsuka, Masato, Sato, Masahiro, Miura, Hiromi, Takabayashi, Shuji, Matsuyama, Makoto, Koyano, Takayuki, Arifin, Naomi, Nakamura, Shingo, Wada, Kenta, Gurumurthy, Channabasavaiah B.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5828090/
https://ncbi.nlm.nih.gov/pubmed/29482575
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-018-1400-x
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados