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GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of embryos
Transgenic technologies used for creating a desired genomic change in animals involve three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them and their subsequent transfer to recipient females. These ex vivo steps have been widely used for over 3 decades and th...
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| Publicado no: | Curr Protoc Hum Genet |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4733652/ https://ncbi.nlm.nih.gov/pubmed/26724720 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142905.hg1508s88 |
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