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Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new
In the recent years, sequence-specific nucleases such as ZFNs, TALENs, and CRISPR/Cas9 have revolutionzed the fields of animal genome editing and transgenesis. However, these new techniques require microinjection to deliver nucleic acids into embryos to generate gene-modified animals. Microinjection...
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| Veröffentlicht in: | Biol Direct |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4815204/ https://ncbi.nlm.nih.gov/pubmed/27037013 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13062-016-0115-8 |
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