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Multiplexed one-step RT-PCR VP7 and VP4 genotyping assays for rotaviruses using updated primers()

The current two-step VP7 and VP4 genotyping RT-PCR assays for rotaviruses have been linked consistently to genotyping failure in an estimated 30% of RVA positive samples worldwide. We have developed a VP7 and VP4 multiplexed one-step genotyping assays using updated primers generated from contemporar...

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Bibliografski detalji
Izdano u:J Virol Methods
Glavni autori: Esona, Mathew D., Gautam, Rashi, Tam, Ka Ian, Williams, Alice, Mijatovic-Rustempasic, Slavica, Bowen, Michael D.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5818709/
https://ncbi.nlm.nih.gov/pubmed/26231786
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2015.07.012
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