Sensitive and Specific Quantitative Detection of Rotavirus A by One-Step Real-Time Reverse Transcription-PCR Assay without Antecedent Double-Stranded-RNA Denaturation

A real-time quantitative reverse transcription-PCR (qRT-PCR) assay using the recombinant thermostable Thermus thermophilus (rTth) enzyme was developed to detect and quantify rotavirus A (RVA). By using rTth polymerase, significant improvement was achieved over the existing real-time RT-PCR assays, w...

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Detalhes bibliográficos
Main Authors: Mijatovic-Rustempasic, Slavica, Tam, Ka Ian, Kerin, Tara K., Lewis, Jamie M., Gautam, Rashi, Quaye, Osbourne, Gentsch, Jon R., Bowen, Michael D.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2013
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3754639/
https://ncbi.nlm.nih.gov/pubmed/23850952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01192-13
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