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Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load()

Techniques such as the real-time reverse transcription-polymerase chain reaction (qRT-PCR) can detect RNA in samples with a low viral load. However, these amplicons typically are either too short or at insufficient concentrations for use in subsequent sequencing reactions for genotyping and detectio...

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Библиографические подробности
Опубликовано в: :J Virol Methods
Главные авторы: Mijatovic-Rustempasic, Slavica, Esona, Mathew D., Williams, Alice L., Bowen, Michael D.
Формат: Artigo
Язык:Inglês
Опубликовано: 2016
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5075964/
https://ncbi.nlm.nih.gov/pubmed/27421626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2016.07.007
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