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Cell-Free Translation of Integral Membrane Proteins into Unilamelar Liposomes

Wheat germ cell-free translation is shown to be an effective method to produce integral membrane proteins in the presence of unilamelar liposomes. In this chapter, we describe the expression vectors, preparation of mRNA, two types of cell-free translation reactions performed in the presence of lipos...

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書目詳細資料
發表在:Methods Enzymol
Main Authors: Goren, Michael A., Nozawa, Akira, Makino, Shin-ichi, Wrobel, Russell L., Fox, Brian G.
格式: Artigo
語言:Inglês
出版: 2009
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5814320/
https://ncbi.nlm.nih.gov/pubmed/19892197
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(09)63037-8
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