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Cell-Free Translation of Integral Membrane Proteins into Unilamelar Liposomes

Wheat germ cell-free translation is shown to be an effective method to produce integral membrane proteins in the presence of unilamelar liposomes. In this chapter, we describe the expression vectors, preparation of mRNA, two types of cell-free translation reactions performed in the presence of lipos...

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Podrobná bibliografie
Vydáno v:Methods Enzymol
Hlavní autoři: Goren, Michael A., Nozawa, Akira, Makino, Shin-ichi, Wrobel, Russell L., Fox, Brian G.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5814320/
https://ncbi.nlm.nih.gov/pubmed/19892197
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(09)63037-8
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