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Cell-Free Translation of Integral Membrane Proteins into Unilamelar Liposomes
Wheat germ cell-free translation is shown to be an effective method to produce integral membrane proteins in the presence of unilamelar liposomes. In this chapter, we describe the expression vectors, preparation of mRNA, two types of cell-free translation reactions performed in the presence of lipos...
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| Publicado no: | Methods Enzymol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5814320/ https://ncbi.nlm.nih.gov/pubmed/19892197 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(09)63037-8 |
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