Activity-Related Microsecond Dynamics Revealed by Temperature-Jump Förster Resonance Energy Transfer Measurements on Thermophilic Alcohol Dehydrogenase

Previous studies of a thermophilic alcohol dehydrogenase (ht-ADH) demonstrated a range of discontinuous transitions at 30°C that include catalysis, kinetic isotope effects, protein hydrogen-deuterium exchange rates, and intrinsic fluorescence properties. Using the FRET response from a Trp-NADH donor...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:J Am Chem Soc
Egile Nagusiak: Vaughn, Morgan B., Zhang, Jianyu, Spiro, Thomas G., Dyer, R. Brian, Klinman, Judith P.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2018
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC5802873/
https://ncbi.nlm.nih.gov/pubmed/29323490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.7b12369
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