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Active Site Hydrophobic Residues Impact Hydrogen Tunneling Differently in a Thermophilic Alcohol Dehydrogenase at Optimal vs. Non-Optimal Temperatures

A growing body of data suggests that protein motion plays an important role in enzyme catalysis. Two highly conserved hydrophobic active site residues in the cofactor-binding pocket of ht-ADH (Leu176 and V260) have been mutated to a series of hydrophobic side chains of smaller size, as well as one d...

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Bibliografiset tiedot
Päätekijät: Nagel, Zachary D., Meadows, Corey W., Dong, Ming, Bahnson, Brian J., Klinman, Judith P.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2012
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Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3498984/
https://ncbi.nlm.nih.gov/pubmed/22568562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi3001352
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