Picosecond-Resolved Fluorescent Probes at Functionally Distinct Tryptophans within a Thermophilic Alcohol Dehydrogenase: Relationship of Temperature-Dependent Changes in Fluorescence to Catalysis

[Image: see text] Two single-tryptophan variants were generated in a thermophilic alcohol dehydrogenase with the goal of correlating temperature-dependent changes in local fluorescence with the previously demonstrated catalytic break at ca. 30 °C (Kohen et al., Nature1999, 399, 496). One tryptophan...

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Main Authors: Meadows, Corey W., Ou, Ryan, Klinman, Judith P.
Format: Artigo
Jezik:Inglês
Izdano: American Chemical Society 2014
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4056859/
https://ncbi.nlm.nih.gov/pubmed/24892947
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jp500825x
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