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Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells

The bioluminescence resonance energy transfer (BRET) assay can be used as an indicator of molecular approximation and/or interaction. A significant resonance energy transfer signal is generated when the acceptor, having the appropriate spectral overlap with the donor emission, is approximated with t...

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Bibliografske podrobnosti
izdano v:Bio Protoc
Main Authors: Harikumar, Kaleeckal G., Yan, Yan, Xu, Ting-Hai, Melcher, Karsten, Xu, H.Eric, Miller, Laurence J.
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5800419/
https://ncbi.nlm.nih.gov/pubmed/29423426
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2904
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