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Type-3 BRET, an Improved Competition-Based Bioluminescence Resonance Energy Transfer Assay
We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membrane...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4070276/ https://ncbi.nlm.nih.gov/pubmed/24940791 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.04.061 |
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