Type-3 BRET, an Improved Competition-Based Bioluminescence Resonance Energy Transfer Assay

We show that in conventional, competition-based bioluminescence resonance energy transfer (BRET) assays of membrane protein stoichiometry, the presence of competitors can alter tagged-protein density and artifactually reduce energy transfer efficiency. A well-characterized monomeric type I membrane...

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Autors principals: Felce, James H., Knox, Rachel G., Davis, Simon J.
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2014
Matèries:
Biophysical Letter
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4070276/
https://ncbi.nlm.nih.gov/pubmed/24940791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.04.061
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