A novel fluorescence assay for measuring phosphatidylserine decarboxylase catalysis

Phosphatidylserine decarboxylases (PSDs) are central enzymes in phospholipid metabolism that produce phosphatidylethanolamine (PE) in bacteria, protists, plants, and animals. We developed a fluorescence-based assay for selectively monitoring production of PE in reactions using a maltose-binding prot...

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Vydáno v:J Biol Chem
Hlavní autoři: Choi, Jae-Yeon, Surovtseva, Yulia V., Van Sickle, Sam M., Kumpf, Jan, Bunz, Uwe H. F., Ben Mamoun, Choukri, Voelker, Dennis R.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2018
Témata:
Methods and Resources
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5798280/
https://ncbi.nlm.nih.gov/pubmed/29247006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA117.000525
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