A practical solution for preserving single cells for RNA sequencing

The design and implementation of single-cell experiments is often limited by their requirement for fresh starting material. We have adapted a method for histological tissue fixation using dithio-bis(succinimidyl propionate) (DSP), or Lomant’s Reagent, to stabilise cell samples for single-cell transc...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Attar, Moustafa, Sharma, Eshita, Li, Shuqiang, Bryer, Claire, Cubitt, Laura, Broxholme, John, Lockstone, Helen, Kinchen, James, Simmons, Alison, Piazza, Paolo, Buck, David, Livak, Kenneth J., Bowden, Rory
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5794922/
https://ncbi.nlm.nih.gov/pubmed/29391536
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-20372-7
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