Improved workflows for high throughput library preparation using the transposome-based nextera system

BACKGROUND: The Nextera protocol, which utilises a transposome based approach to create libraries for Illumina sequencing, requires pure DNA template, an accurate assessment of input concentration and a column clean-up that limits its applicability for high-throughput sample preparation. We addresse...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:BMC Biotechnol
Main Authors: Lamble, Sarah, Batty, Elizabeth, Attar, Moustafa, Buck, David, Bowden, Rory, Lunter, Gerton, Crook, Derrick, El-Fahmawi, Bassam, Piazza, Paolo
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2013
Teme:
Methodology Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4222894/
https://ncbi.nlm.nih.gov/pubmed/24256843
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-104
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