Bead-linked transposomes enable a normalization-free workflow for NGS library preparation

Abstract Background Transposome-based technologies have enabled the streamlined production of sequencer-ready DNA libraries; however, current methods are highly sensitive to the amount and quality of input nucleic acid. Results We describe a new library preparation technology (Nextera DNA Flex) that...

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Autori principali: Stephen Bruinsma, Joshua Burgess, Daniel Schlingman, Agata Czyz, Natalie Morrell, Catherine Ballenger, Heather Meinholz, Lee Brady, Anupama Khanna, Lindsay Freeberg, Rosamond G Jackson, Pascale Mathonet, Susan C Verity, Andrew F Slatter, Rooz Golshani, Haiying Grunenwald, Gary P Schroth, Niall A Gormley
Natura: Artigo
Lingua:Inglês
Pubblicazione: BMC 2018-10-01
Serie:BMC Genomics
Soggetti:
Next-generation sequencing
Library preparation
Transposome
Accesso online:http://link.springer.com/article/10.1186/s12864-018-5096-9
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