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One-Pot Enzymatic Glycan Remodeling of a Therapeutic Monoclonal Antibody by Endoglycosidase S (Endo-S) From Streptococcus pyogenes

A facile, one-pot enzymatic glycan remodeling of antibody rituximab to produce homogeneous high-mannose and hybrid type antibody glycoforms is described. This method was based on the unique substrate specificity of the endoglycosidase S (Endo-S) from Streptococcus pyogenes. While Endo-S efficiently...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Bioorg Med Chem
Main Authors: Tong, Xin, Li, Tiezheng, Orwenyo, Jared, Toonstra, Christian, Wang, Lai-Xi
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5788734/
https://ncbi.nlm.nih.gov/pubmed/28789910
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmc.2017.07.053
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