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Site-specific immobilization of endoglycosidases for streamlined chemoenzymatic glycan remodeling of antibodies

Chemoenzymatic glycan remodeling of antibodies using an endoglycosidase and its mutant is emerging as an attractive approach for producing homogeneous antibody glycoforms. We report in this paper a site-specific covalent immobilization of the endoglycosidases (Endo-S2 and its glycosynthase mutant D1...

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Detalhes bibliográficos
Publicado no:Carbohydr Res
Main Authors: Li, Tiezheng, Li, Chao, Quan, David N., Bentley, William E., Wang, Lai-Xi
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5837956/
https://ncbi.nlm.nih.gov/pubmed/29475193
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.carres.2018.02.007
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