Large‐scale image‐based profiling of single‐cell phenotypes in arrayed CRISPR‐Cas9 gene perturbation screens

High‐content imaging using automated microscopy and computer vision allows multivariate profiling of single‐cell phenotypes. Here, we present methods for the application of the CISPR‐Cas9 system in large‐scale, image‐based, gene perturbation experiments. We show that CRISPR‐Cas9‐mediated gene pertur...

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Bibliografiska uppgifter
I publikationen:Mol Syst Biol
Huvudupphovsmän: de Groot, Reinoud, Lüthi, Joel, Lindsay, Helen, Holtackers, René, Pelkmans, Lucas
Materialtyp: Artigo
Språk:Inglês
Publicerad: John Wiley and Sons Inc. 2018
Ämnen:
Methods
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5787707/
https://ncbi.nlm.nih.gov/pubmed/29363560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/msb.20178064
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