Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells

Quantitative gene expression analysis in intact single cells can be achieved using single molecule-based fluorescence in situ hybridization (smFISH). This approach relies on fluorescence intensity to distinguish between true signals, emitted from an RNA copy hybridized with multiple oligonucleotide...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Main Authors: Cui, Yi, Hu, Dehong, Markillie, Lye Meng, Chrisler, William B, Gaffrey, Matthew J, Ansong, Charles, Sussel, Lori, Orr, Galya
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2018
Assuntos:
Methods Online
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5778465/
https://ncbi.nlm.nih.gov/pubmed/29040675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx874
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