Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells

Quantitative gene expression analysis in intact single cells can be achieved using single molecule-based fluorescence in situ hybridization (smFISH). This approach relies on fluorescence intensity to distinguish between true signals, emitted from an RNA copy hybridized with multiple oligonucleotide...

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Cui, Yi, Hu, Dehong, Markillie, Lye Meng, Chrisler, William B, Gaffrey, Matthew J, Ansong, Charles, Sussel, Lori, Orr, Galya
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2018
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5778465/
https://ncbi.nlm.nih.gov/pubmed/29040675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx874
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