Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells

Quantitative gene expression analysis in intact single cells can be achieved using single molecule-based fluorescence in situ hybridization (smFISH). This approach relies on fluorescence intensity to distinguish between true signals, emitted from an RNA copy hybridized with multiple oligonucleotide...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Cui, Yi, Hu, Dehong, Markillie, Lye Meng, Chrisler, William B, Gaffrey, Matthew J, Ansong, Charles, Sussel, Lori, Orr, Galya
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2018
Fag:
Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5778465/
https://ncbi.nlm.nih.gov/pubmed/29040675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx874
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