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Folding of maltose binding protein outside of and in GroEL

We used hydrogen exchange–mass spectrometry (HX MS) and fluorescence to compare the folding of maltose binding protein (MBP) in free solution and in the GroEL/ES cavity. Upon refolding, MBP initially collapses into a dynamic molten globule-like ensemble, then forms an obligatory on-pathway native-li...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Ye, Xiang, Mayne, Leland, Kan, Zhong-yuan, Englander, S. Walter
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5776991/
https://ncbi.nlm.nih.gov/pubmed/29295923
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1716168115
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