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Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm
BACKGROUND: The beauty and power of the genome editing mechanism, CRISPR Cas9 endonuclease system, lies in the fact that it is RNA-programmable such that Cas9 can be guided to any genomic loci complementary to a 20-nt RNA, single guide RNA (sgRNA), to cleave double stranded DNA, allowing the introdu...
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| Publicado no: | BMC Genomics |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5773898/ https://ncbi.nlm.nih.gov/pubmed/29219081 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-017-4225-1 |
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