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Development of versatile non-homologous end joining-based knock-in module for genome editing

CRISPR/Cas9-based genome editing has dramatically accelerated genome engineering. An important aspect of genome engineering is efficient knock-in technology. For improved knock-in efficiency, the non-homologous end joining (NHEJ) repair pathway has been used over the homology-dependent repair pathwa...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Sawatsubashi, Shun, Joko, Yudai, Fukumoto, Seiji, Matsumoto, Toshio, Sugano, Shigeo S.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5766535/
https://ncbi.nlm.nih.gov/pubmed/29330493
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-18911-9
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