Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes

Genome editing using the CRISPR/Cas9 system requires the presence of guide RNAs bound to the Cas9 endonuclease as a ribonucleoprotein (RNP) complex in cells, which cleaves the host cell genome at sites specified by the guide RNAs. New genetic material may be introduced during repair of the double-st...

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Bibliografiska uppgifter
I publikationen:Methods
Huvudupphovsmän: Jacobi, Ashley M., Rettig, Garrett R., Turk, Rolf, Collingwood, Michael A., Zeiner, Sarah A., Quadros, Rolen M., Harms, Donald W., Bonthuis, Paul J., Gregg, Christopher, Ohtsuka, Masato, Gurumurthy, Channabasavaiah B., Behlke, Mark A.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2017
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Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5761324/
https://ncbi.nlm.nih.gov/pubmed/28351759
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2017.03.021
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