Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli

Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive aggregates of misfolded protein, and thus should be avoided during recombinant protein production, numerous biopharmaceutically important proteins are currently produced as IBs. To obtain correctly folded,...

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Detalhes bibliográficos
Publicado no:Appl Microbiol Biotechnol
Main Authors: Wurm, David J., Quehenberger, Julian, Mildner, Julia, Eggenreich, Britta, Slouka, Christoph, Schwaighofer, Andreas, Wieland, Karin, Lendl, Bernhard, Rajamanickam, Vignesh, Herwig, Christoph, Spadiut, Oliver
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2017
Assuntos:
Biotechnological Products and Process Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5756567/
https://ncbi.nlm.nih.gov/pubmed/29159587
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-017-8641-6
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