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Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy

The bacterium E. coli is one of the most important hosts for recombinant protein production. The benefits are high growth rates, inexpensive media, and high protein titers. However, complex proteins with high molecular weight and many disulfide bonds are expressed as inclusion bodies (IBs). In the l...

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Bibliographic Details
Published in:Int J Mol Sci
Main Authors: Schwaighofer, Andreas, Ablasser, Sarah, Lux, Laurin, Kopp, Julian, Herwig, Christoph, Spadiut, Oliver, Lendl, Bernhard, Slouka, Christoph
Format: Artigo
Language:Inglês
Published: MDPI 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7313074/
https://ncbi.nlm.nih.gov/pubmed/32485932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21113881
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