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Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy

The bacterium E. coli is one of the most important hosts for recombinant protein production. The benefits are high growth rates, inexpensive media, and high protein titers. However, complex proteins with high molecular weight and many disulfide bonds are expressed as inclusion bodies (IBs). In the l...

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Bibliografske podrobnosti
izdano v:Int J Mol Sci
Main Authors: Schwaighofer, Andreas, Ablasser, Sarah, Lux, Laurin, Kopp, Julian, Herwig, Christoph, Spadiut, Oliver, Lendl, Bernhard, Slouka, Christoph
Format: Artigo
Jezik:Inglês
Izdano: MDPI 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7313074/
https://ncbi.nlm.nih.gov/pubmed/32485932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21113881
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