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Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy

The bacterium E. coli is one of the most important hosts for recombinant protein production. The benefits are high growth rates, inexpensive media, and high protein titers. However, complex proteins with high molecular weight and many disulfide bonds are expressed as inclusion bodies (IBs). In the l...

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Pubblicato in:Int J Mol Sci
Autori principali: Schwaighofer, Andreas, Ablasser, Sarah, Lux, Laurin, Kopp, Julian, Herwig, Christoph, Spadiut, Oliver, Lendl, Bernhard, Slouka, Christoph
Natura: Artigo
Lingua:Inglês
Pubblicazione: MDPI 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7313074/
https://ncbi.nlm.nih.gov/pubmed/32485932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21113881
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