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Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

The integrase (IN) protein of the retrovirus prototype foamy virus (PFV) is a model enzyme for studying the mechanism of retroviral integration. Compared to IN from other retroviruses, PFV IN is more soluble and more amenable to experimental manipulation. Additionally, it is sensitive to clinically...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Lopez, Miguel A., Mackler, Randi M., Altman, Matthew P., Yoder, Kristine E.
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755535/
https://ncbi.nlm.nih.gov/pubmed/29286489
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56605
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