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Removal of nuclease contamination during purification of recombinant Prototype Foamy Virus integrase
Retroviral infection requires integration of the viral genome into the host genome. Recombinant integrase proteins may be purified following bacterial expression. A bulk biochemical assay of integrase function relies on the conversion of supercoiled plasmids to linear or relaxed circles. Single mole...
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| Publicado no: | J Virol Methods |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4992616/ https://ncbi.nlm.nih.gov/pubmed/27269588 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2016.06.002 |
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